Browsing by Subject "576"
Now showing items 1-20 of 586
A 3D culture system to study gene regulation in breast cancer cells
In this Thesis we report the successful growth of hormone responsive T47D cells as 3D spheroids in Matrigel mimicking the ECM. A detailed characterization by using a genome-wide approach of the main physical and biochemical ...
A functional study of the conserved LSM proteins in C. elegans reveals their involvement in the stress response of metazoans
Lsm proteins regulate RNA metabolism and are conserved in the three domains of life, typically functioning as RNA-binding complexes involved in a wide range of post-transcriptional mechanisms. Generally, their functions ...
A human pancreatic ribonuclease variant kills cancer cells by apoptosis and reduces the expression of P-glycoprotein in MDR cell lines
En aquesta tesi s'han estudiat les propietats antitumorals d'una variant de la ribonucleasa pancreàtica humana anomenada PE5 que incorpora un senyal de localització nuclear. Aquest estudi mostra que PE5 indueix l'apoptosi ...
A mechano-perfusion bioreactor for tissue engineering
Tissue Engineering plays a vital role in tissue construct to repair, maintain or replace tissues. Those tissues can be cultivated in vivo or in vitro using devices such as bioreactors. There are several approaches to ...
A midzone-based ruler adjusts chromosome compaction to analphase spindle length
Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation ...
A new experimental model to study bone and cartilage formation using a bioengineering approach
La medicina regenerativa és una disciplina que ha guanyat reconeixement en les últimes dècades pel fet que moltes malalties no són tractables amb fàrmacs convencionals. Molts grups de recerca i empreses inverteixen temps ...
A practical approach on boar sperm cryodamage. Morphofunctional and immunocytochemical study of cryopreserved boar sperm intended for use in artificial insemination
L'ús d'esperma criopreservada en la inseminació artificial (IA) d'espècies d'interès productiu permet un major control sanitari i la creació de bancs de germoplasma d'alt valor genètic, entre d'altres avantatges. En el ...
A. C. elegans model for X-linked adrenoleukodystrophy : roles of pmp-4 fatty acid transporter in the nervous system
X-linked adrenoleukodystrophy (X-ALD) is an inherited neurodegenerative disorder. The genetic bases for all its different phenotypic variants are mutation in the gene encoding the peroxisomal ATP-binding cassette (ABC) ...
Activació i regulació de la quinasa Srk1 en resposta a estrès en "Schizosaccharomyces pombe"
Les cèl·lules de llevat, al igual que les cèl·lules eucariotes d'organismes superiors, responen a situacions d'estrès extracel·lular activant la via de les MAP quinases, les quals transdueixen el senyal activant l'expressió ...
Adipose tissue remodelling by polyphenols in obese rats
Els trastorns metabòlics vinculats a l’obesitat es correlaciona amb una disfunció en la capacitat d’expansió del teixit adipós blanc (TAB), la qual es produeix a través de la hipertròfia (augment de mida d’adipòcits) i/o ...
Advanced physiomimetic models for in vitro studies on respiratory diseases
[spa] Las células se encuentran en un entorno concreto, específico para la función de cada órgano. En el caso de los pulmones, la matriz extracelular ha de tener unas propiedades físicas que les permitan soportar los ...
Advanced single molecule fluorescent tools to reveal spatiotemporal multi-molecular interactions in living cells
(English) The spatiotemporal organisation and compartmentalisation of molecules in living cells is crucial to regulate cell function. Dysregulation in how molecules dynamically explore their environment and interact with ...
Algoritme diagnòstic en el laboratori. Impacte dels nous índex hematimètrics
Currently, automatic analyzers based on highly accurate methods can determine hematologic parameters in a fast and reliable way which is important to detect abnormalities in blood cells. In laboratories with a high care ...
Alteraciones de los Astrocitos en el envejecimiento y en la enfermedad del Alzheimer
El tema de esta tesis ha sido el estudio de los mecanismos de neurodegeneración asociados al envejecimiento cerebral y el papel que desempeñan los astrocitos como neuroprotectores utilizando modelos animales in vitro e in ...
Alternative splicing dynamics during somatic cell reprogramming
Alternative splicing (AS) is a mechanism of post-transcriptional regulation that plays an important role during development and in the maintenance of stem cell pluripotency. The dynamics of AS regulation during somatic ...
Analysis of CK2-dependent regulation of Myo5-induced actin polymerization during the endocytic uptake in S. Cerevisiae / Análisis de la regulación mediada por CK2 de la polimerización de actina inducida por Myo5 durante la internalización endocítica en “S. Cerevisiae”
S. cerevisiae (budding yeast) is particularly powerful to study the relation between the molecular mechanisms of actin regulation and its biological significance due to its powerful molecular and genetic methods, ...
Analysis of Reelin function in the molecular mechanisms underlying Alzheimer’s disease
Alzheimer’s disease (AD) is the most common cause of dementia in the elderly, with more than 25 million people affected worldwide. First described in 1907 by the German physician Alois Alzheimer, AD is a neurodegenerative ...
Analysis of the contribution of Barrier-to-Autointegration Factor (BAF) to centromere function and mitosis progression
This work is focused on Barrier-to-Autointegration Factor (BAF) protein. BAF is a nuclear envelope (NE) component that binds chromatin and is required for NE reassembly (NER) at mitosis exit. Previous work in our group ...
Analysis of the molecular function of kazrin in endosomal trafficking
Endocytosis and endosomal trafficking are of central meaning in all eukaryotic cells. It enables cells to rapidly modify their surface exposure of signalling complexes, nutrient and ion transporters, to maintain the ...
Anàlisi bioquímica de l’interactoma de MLC1 i GlialCAM: impacte en la Leucoencefalopatia Megalencefàlica
El propòsit principal d’aquesta tesi és obtenir nous coneixements sobre la fisiopatologiade la Leucoencefalopatia Megalencefàlica amb Quists subcorticals (MLC, de l’anglèsMegalencephalic Leukoencephalopathy with subcorticals ...
